The effect of high-intensity interval training (HIIT) on resistin gene expression in visceral adipose tissue in obese male rats

  • Elham Jamali Graduate student of Exercise Physiology in Health, University of Payam Noor, Karaj, Iran
  • Mohamamd Reza Asad Department of Exercise Physiology, University of Payam Noor, Karaj, Iran
  • Ali Rasoli Department of Pharmacology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.


It is has been reported that obesity can result in the accumulation of various proinflammatory factors such as resistin in adipose tissue. This study aimed to investigate the effect of HIIT on resistin gene expression in visceral adipose tissues in diet-induced obese rats. Nineteen male rats were used in the present study. 6 rats (control group) were fed with standard diet for 8 weeks and then dissected and their visceral adipose tissues were sampled. At the same time, 13 rats were fed with a high-fat diet for 8 weeks. From these obese rats, 6 animals (fat group) were fed a high-fat diet for another 8 weeks and 7 animals (training group), underwent HIIT for 8 weeks. Training included running on a treadmill at 90% VO2max with 5 repetitions of 30 seconds in each session at 29 to 36 meters per minute with one-minute active rest intervals. The latter two groups were also dissected and their visceral adipose tissues were sampled. Measurement of resistin gene expression in tissue samples was done using real-time polymerase chain reaction. Data were analysed with one-way ANOVA. Obesity increased resistin gene expression in rats 2-fold but it was not statistically significant (P= 0.618). HIIT reduced resistin gene expression 8-fold compared to the fat group, but this reduction was not significant, too (P= 0.349). In conclusion, HIIT could reduce resistin gene expression as a proinflammatory factor in the visceral adipose tissue of obese rat. Despite the non-significance of differences, resistin gene expression was reduced several folds, so further studies are needed in this field.
  Keywords: high intense interval training (HIIT), resistin, gene expression, visceral fat, rat


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1. World Health Organization. Global status report on noncommunicable diseases 2010. Geneva .WHO, 2011; 18-19; 49-52.
2. Slentz CA, Houmard JA, Krouse WE. Exercise abdominal obesity skeletal muscle and metabolic risk: evidence of a dose response. Obesity 2009; 3(Suppl.): 27-33.
3. Ahima RS, Flier JS. Adipose tissue as an endocrine organ. Trends Endocrinal Metab 2000 ; 11: 327-332.
4. Ibrahim MM. Subcutaneous and visceral adipose tissue: structural and functional difference. Obesity Rev 2009; 11(1): 11-18.
5. Henry SL, Bensly JG. White adipocytes more than just depots. Int Biochem Obes 2010; 44: 435-440.
6. Ridker PK, Hennekes CH, Buring JE. CRP and other markers of inflammation in the prediction of cardiovascular disease in woman. N Engl J Med 2000; 23: 836-843.
7. Fantuzzi, G, Adipose tissue, adipokines, and inflammation. J. Allergy Clin Immunol.2005; 115(5): 911-919.
8. Lago F, Gomes R, Conde J, Scotece M, Dieguez C, Guilillo O. Functions of adipose tissue and adipokines in health and disease. In: Aimaretti G, Marzullo P, Prodam F. (Eds) Update on mechanisms of hormone action: focus on metabolism, growth and reproduction. 2011; Pub: Intech open access.
9. Galic S, Oakhill JS. Adipose tissue as an endocrine organ. Moll Cell Endoc 2010; 316; 129-139.
10. Adeghate E. An update on the biology and physiology of resistin. Cell Mol Life Sci 2004; 61 (19-20) :2485-2496.
11. Sloan R, Shapiro A, Ronald E, Paula S, Exercise inflammation and heart disease risk. J. Appl. Physiol, 2007; 103: 1007-1011.
12. Haghighi AH. The effect of aerobic exercise on serum resistin and adiponectin and anthropometric variables obese women. Olympic Quarterly 1392; 21 (1): 61-76[Persian].
13. Matthew B, Miller G, The effect of the short term-high intensity circuit training program on work capacity, body composition, and blood profiles in sedentary obese men: a pilot study. Biomed. Res. Intl, 2014; Article ID. 191797.
14. Giballa MJ, Mc Gee SL. Metabolic adaptation to short- term HIIT: a little pain for a lot of pain. Exerc Sport Sci Rev 2008; 36 (2): 58-63.
15. Burgmaster KA, Heigenhazer GJ, Gibala MJ. Effect of short term interval training on human skeletal muscle carbohydrate metabolism during exercise and time trial performance. J Appl Physiol 2006; 100: 2041-2044.
16. Babraj JA, Volard NB. Extremely short duration high intensity training substantially improves insulin action in young health males. BMC Endocr Disord 2009; 9: 1-8.
17. Ciolac EG. HIIT and hypertension maximizing the benefits of exercise: review article. Am J Cardiovasc 2012; 2 (2): 102-110.
18. Zhang M, Lv XY, Li J, Xu ZG, Chen L. The characterization of high-fat diet and multiple low-dose streptozotocin induced type 2 diabetes rat model. Exp diabetes Res 2008; article ID: 704045.
19. Lee MO. Determination of the surface area of the white rat with its application to the impression of metabolic results. AM J physiol 1929; 89: 24-33.
20. Kim K, Kim YH, Lee SH. Effect of exercise intensity on folded protein response in skeletal muscle of rat. Korean J Physiol Pharmacol 2014; 18: 211-216.
21. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real- time quantitative PCR and the 2)^-ΔΔCt) method. Methods 2001; 25(4): 402-408.
22. Rungvaux A, Shea RJ. Pre B-cell colony enhancing factor, whose expression is up-regulated in activated lymphocytes in a nicotinamide phosphoryl transferase a cytosolic enzyme involved in DNA biosynthesis. Eur J Immunol 2002; 32: 3225-3234.
23. Junes T, Basilio J. Long term exercise training in over weight adolescents improves plasma peptide YY and resistin. Obesity 2009; 17: 1189-1195.
24. Bhutani S, Klempel MC, Beregr RA. Improvements in coronary heart disease risk indicators by alternate day fasting involve adipose tissue modulations. Obesity 2010; 18: 2152- 2159.
25. Xu W, Yu L. Resistin increased lipid accumulation and CD36 expression in human macrophage. Biochem Biophys Res Commun 2006; 351 (2): 376-382.
26. Rajala MW, Lyn Y. Cell type specific expression and co-regulation of murine resistin and resistin-like molecule-alpha in adipose tissue. Mol Endocrinol 2002; 16: 1920-1930.
27. Pedersen BK, Saltin B. Evidence for prescribing exercise as therapy in chronic disease. Scand. J Med Sci Sports 2006; 16 (1): 53-63.
28. Yannakoulia M, Yiannakouris N, Bluher S, Matalas AL. Body fat mass and macro nutrient intake in relation to circulating soluble leptin receptor, free leptin index, adiponectin, and resistin concentration in healthy human. J Clin Endocrinal Metab 2003; 88:1730-1736.
29. Kadoglou N, Perrea D, Iliadis F, Angelopoulou N, Liapis C, Alevizos M. Exercise reduces resistin and inflammatory cytokines in patients with type 2 diabetes. Diabetes care 2007; 30 (3): 719-729.
30. Balducci S, Zanuso S, Nicolucci A, Fernando F, Cavallo S, Cardelli, P, et al. Anti-inflammatory effect of exercise training in subjects with type 2 diabetes and the metabolic syndrome is dependent on exercise modalities and independent of weight loss. Nutr Metab Cardiovasc Dis 2010; 20 (8): 608-617.
31. Rashid Lamir A, Gholamian S, Atri A. The effect of aerobic exercise on resistin gene expression in active young women. J Exerc Physiol 2013; 18: 95-106. [Persian].
32. Dastani M, Rashid Lamir A, Rashid Lamir S. The effect of 8 weeks of earobic training on hs CRP and resistin level in menopaused woman. Eu J Biol 2013; 3(4): 43- 44.
How to Cite
Jamali, E., Asad, M. R., & Rasoli, A. (2016). The effect of high-intensity interval training (HIIT) on resistin gene expression in visceral adipose tissue in obese male rats. International Journal of Applied Exercise Physiology, 5(1), 17-25. Retrieved from
Applied Exercise Physiology