Alpha-Actinin-3 (ACTN3) R/X Gene Polymorphism and Physical Performance of Multi-Ethnic Malaysian Population

ACTN3 R/X gene polymorphism in multi-ethnic Malaysian population

  • Hazwani Ahmad Yusof 1Lifestyle Science Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Pulau Pinang, Malaysia
  • Rabindarjeet Singh 1Lifestyle Science Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Pulau Pinang, Malaysia
  • Zafarina Zainuddin 2Analytical Biochemistry Research Centre, Universiti Sains Malaysia, 11800 Universiti Sains Malaysia, Pulau Pinang, Malaysia
  • Kieron Rooney Discipline of Exercise and Sport Science, University of Sydney, New South Wales, 2006, Australia
  • Ahmad Munir Che Muhamed Lifestyle Science Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Pulau

Abstract

A disparity population data set in the current literature with limited reports among Asian samples and the inconsistent findings among different ethnic groups warrant further investigation on the association between alpha-actinin-3 (ACTN3) R/X gene polymorphism and human physical performance in Asian population. This study was designed to examine the association between ACTN3 R/X gene polymorphism and physical performance of multi-ethnic Malaysian population. One hundred eighty well-trained athletes (34 endurance, 41 strength, and 105 intermittent) and 180 controls were drawn from four ethnic groups in Malaysia (Malay, Chinese, Indian, and Other Bumiputra). A sample of deoxyribonucleic acid (DNA) was retrieved from a buccal swab from each participant and the ACTN3 R/X genotype was identified through polymerase chain reaction and restriction fragment length polymorphism analysis. The strength and endurance performances of the athletes were evaluated with maximal voluntary contraction and Yo-Yo intermittent recovery level 2 tests, respectively. The independent t-test, chi-square, multivariate, and one-way analysis of variance were used for data analysis. ACTN3 R/X alleles (p = 0.672) and genotype (p = 0.355) frequencies did not vary much between the multi-ethnic groups of Malaysian athletes. These small variations did not have any influence on handgrip strength (p = 0.334), leg strength (p = 0.256), and Yo-Yo intermittent recovery level 2 performance (p = 0.425) between these ethnic groups. The RR and XX genotypes were more frequent among strength and intermittent athletes, respectively. Athletes with the RR genotype had greater handgrip than those with the RX genotype (p = 0.031), but not different from athletes with the XX genotype (p = 0.228). Athletes with RR genotype also have a higher leg strength than those with the RX (p = 0.001) and XX genotype (p = 0.010). However, the endurance performance was similar among genotype groups (p = 0.385). The ACTN3 R/X gene polymorphism may confer similar effect on physical performance across different ethnic groups in Malaysia with the R allele was associated with greater strength performance whilst the X allele was not associated with endurance performance of multi-ethnic Malaysian population.

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Published
2016-09-30
How to Cite
Ahmad Yusof, H., Singh, R., Zainuddin, Z., Rooney, K., & Munir Che Muhamed, A. (2016). Alpha-Actinin-3 (ACTN3) R/X Gene Polymorphism and Physical Performance of Multi-Ethnic Malaysian Population. International Journal of Applied Exercise Physiology, 5(3), 18-30. Retrieved from http://www.ijaep.com/index.php/IJAE/article/view/78
Section
Applied Exercise Physiology